Selected primary antibody| Cleaved Caspase-3 antibody recommendation

Date：2019-12-12 Views：1853

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Cleaved Caspase-3 is an Activated Form of Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.

All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. The main substrate of Caspase-3 is poly (ADP- ribose) polymerase PARP (ADP-ribose) which is related to DNA repair and gene integrity monitoring. When apoptosis is initiated, Caspase-3 is activated by cleavage of Asp-28/Ser-29 (between N-terminal prodomains) and Asp-175/Ser-176 (between large subunit and small subunit), resulting in a large subunit of 17kDa and a small subunit of 12kDa. P17 and p12 dimerize to form cleaved caspase-3 in activated form.

The picture is from https://www.nature.com/articles/cdd2009180.

AbbkineCleaved-Caspase-3 p17 (D175) Polyclonal Antibody helps basic cancer research! Basic information about the product:

Product name	#CAT	Reactivity	Applications	Dilution ratio	Observed Band(KD)
Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody	ABP0021	human/mouse/rat	WB/IF/IHC-P/ELISA	WB (1:500-1:2000) IF (1:50-1:300) IHC-P (1:50-1:300) ELISA（1:20000）	19/17 kD