| Product name | Mouse Postacrosomal sheath WW domain-binding protein (WBP2NL) ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | This Mouse Postacrosomal sheath WW domain-binding protein (WBP2NL) ELISA Kit employs a two-site sandwich ELISA to quantitate WBP2NL in samples. An antibody specific for WBP2NL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWBP2NL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WBP2NL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WBP2NL bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | WBP2NL; CTA-250D10.11; FLJ26145; MGC26816; PAWP; OTTHUMP00000199648; postacrosomal sheath WW domain-binding protein |
| Kit components | • Mouse Postacrosomal sheath WW domain-binding protein microplate • Mouse Postacrosomal sheath WW domain-binding protein standard • Mouse Postacrosomal sheath WW domain-binding protein detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Mouse Postacrosomal sheath WW domain-binding protein (WBP2NL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse WBP2NL. No significant cross-reactivity or interference between Mouse WBP2NL and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | WBP4 encodes WW domain-containing binding protein 4. The WW domain represents a small and compact globular structure that interacts with proline-rich ligands. This encoded protein is a general spliceosomal protein that may play a role in cross-intron bridging of U1 and U2 snRNPs in the spliceosomal complex A. The predicted 376-amino acid FBP21 protein contains an N-terminal zinc finger motif and 2 WW domains. Western blot analysis of in vitro translated FBP21 protein and of HeLa cell extracts showed that FBP21 migrates as a 58-kD polypeptide. Immunofluorescence studies of HeLa cells indicated that endogenous FBP21 is exclusively localized to the nucleus and concentrated in a punctate pattern. In addition, FBP21 colocalized with the essential pre-mRNA splicing factor SC35. |
| Gene ID | 74716 |
| Alternative | WBP2NL; CTA-250D10.11; FLJ26145; MGC26816; PAWP; OTTHUMP00000199648; postacrosomal sheath WW domain-binding protein |
| Accession | Q9D529 |
Fig.1. Mouse Postacrosomal sheath WW domain-binding protein (WBP2NL) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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