| Product name | Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2K in samples. An antibody specific for UBE2K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2K is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2K bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | UBE2K; DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; huntingtin interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K; ubiquitin-p |
| Kit components | • Human Ubiquitin-conjugating enzyme E2 K microplate • Human Ubiquitin-conjugating enzyme E2 K standard • Human Ubiquitin-conjugating enzyme E2 K detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2K. No significant cross-reactivity or interference between Human UBE2K and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. The 200-amino acid HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K. Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. Western blot analysis detected protein bands of 25, 28, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system. |
| Gene ID | 3093 |
| Alternative | UBE2K; DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; huntingtin interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K; ubiquitin-p |
| Accession | P61086 |
Fig.1. Human Ubiquitin-conjugating enzyme E2 K (UBE2K) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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