| Product name | Human PHD finger protein 7 (PHF7) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human PHD finger protein 7 (PHF7) ELISA Kit employs a two-site sandwich ELISA to quantitate PHF7 in samples. An antibody specific for PHF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHF7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHF7 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PHF7; DKFZp434L1850; HSPC045; HSPC226; MGC26088; NYD-SP6; |
| Kit components | • Human PHD finger protein 7 microplate • Human PHD finger protein 7 standard • Human PHD finger protein 7 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human PHD finger protein 7 (PHF7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PHF7. No significant cross-reactivity or interference between Human PHF7 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, PHF7 is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. |
| Gene ID | 51533 |
| Alternative | PHF7; DKFZp434L1850; HSPC045; HSPC226; MGC26088; NYD-SP6; |
| Accession | Q9BWX1 |
Fig.1. Human PHD finger protein 7 (PHF7) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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