| Product name | Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM5 in samples. An antibody specific for TOMM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM5 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | TOMM5; RP11-613M10.3; C9orf105; RP11-263I4.1; Tom5; bA613M10.3; mitochondrial outer membrane protein TOM5; translocase of outer mitochondrial membrane 5 homolog |
| Kit components | • Human Mitochondrial import receptor subunit TOM5 homolog microplate • Human Mitochondrial import receptor subunit TOM5 homolog standard • Human Mitochondrial import receptor subunit TOM5 homolog detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM5. No significant cross-reactivity or interference between Human TOMM5 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which regulate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These results indicate that human small Tom proteins maintain the structural integrity of the TOM complex. |
| Gene ID | 401505 |
| Alternative | TOMM5; RP11-613M10.3; C9orf105; RP11-263I4.1; Tom5; bA613M10.3; mitochondrial outer membrane protein TOM5; translocase of outer mitochondrial membrane 5 homolog |
| Accession | Q8N4H5 |
Fig.1. Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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