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Human MIF Protein

Human MIF Protein

Views(20) Publications(0) Catalog no(PRP1947)
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Specification

Product name Human MIF Protein
Sequence Amino acid sequence derived from Human MIF (P14174) (Met1-Ala115) was expressed.
Applications No tag
Applications notes Human
Activity Testing in progress
Protein length The recombinant Human MIF consists of 120 amino acids and predicts a molecular mass of 13.1 kDa.
Preparation method E.coli
Purity > 97 % as determined by SDS-PAGE
Molecular Formula 13.1 KDa
Alternative GLIF, mMIF, GIF, Glycosylation-inhibiting factor

Product Properties

Formulation Lyophilized from sterile 20mM Tris,150mM NaCl, pH 8.0.
Features & Benefits < 1 EU per μg of the protein as determined by the LAL method.
Molecular weight 13.1 KDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Human MIF is a 12.5 kDa, 115 amino acid nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity.
Gene ID 4282
Alternative GLIF, mMIF, GIF, Glycosylation-inhibiting factor
Accession P14174

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