Product name | Human MIF Protein |
Sequence | Amino acid sequence derived from Human MIF (P14174) (Met1-Ala115) was expressed. |
Applications | No tag |
Applications notes | Human |
Activity | Testing in progress |
Protein length | The recombinant Human MIF consists of 120 amino acids and predicts a molecular mass of 13.1 kDa. |
Preparation method | E.coli |
Purity | > 97 % as determined by SDS-PAGE |
Molecular Formula | 13.1 KDa |
Alternative | GLIF, mMIF, GIF, Glycosylation-inhibiting factor |
Formulation | Lyophilized from sterile 20mM Tris,150mM NaCl, pH 8.0. |
Features & Benefits | < 1 EU per μg of the protein as determined by the LAL method. |
Molecular weight | 13.1 KDa |
Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Storage instructions | Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Human MIF is a 12.5 kDa, 115 amino acid nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity. |
Gene ID | 4282 |
Alternative | GLIF, mMIF, GIF, Glycosylation-inhibiting factor |
Accession | P14174 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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