| Product name | Human Matrix metallopeptidase 25 (MMP25) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Matrix metallopeptidase 25 (MMP25) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP25 in samples. An antibody specific for MMP25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP25 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MMP25; MMP20; MMP20A; MMPL1; MT-MMP6; MT6-MMP; leukolysin; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-like 1; membrane-type 6 matrix metal |
| Kit components | • Human Matrix metallopeptidase 25 microplate • Human Matrix metallopeptidase 25 standard • Human Matrix metallopeptidase 25 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Matrix metallopeptidase 25 (MMP25) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP25. No significant cross-reactivity or interference between Human MMP25 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. |
| Gene ID | 64386 |
| Alternative | MMP25; MMP20; MMP20A; MMPL1; MT-MMP6; MT6-MMP; leukolysin; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-like 1; membrane-type 6 matrix metal |
| Accession | Q9NPA2 |
Fig.1. Human Matrix metallopeptidase 25 (MMP25) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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