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Human IFN-Ω Protein, His tag (Animal-Free)

Human IFN-Ω Protein, His tag (Animal-Free)

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Specification

Product name Human IFN-Ω Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IFN-Ω (Cys24-Ser195) (P05000) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.02 ng/mL. The specific activity of recombinant human IFN omega is approximately >5 x10⁷ IU/ mg.
Protein length The recombinant Human IFN-Ω consists of 172 amino acids and predicts a molecular mass of 20.1 kDa.
Preparation method E.coli
Purity > 95 % as determined by SDS-PAGE
Molecular Formula 20.93 kDa
Alternative interferon omega, IFN alpha II-1, IFNW1

Product Properties

Formulation Lyophilized from sterile PBS,pH 7.4.
Features & Benefits <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 20.93 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interferon Omega (IFN-ω) is a 20.12 kDa member of type I IFN family with 173 amino acid residues. IL-28B is expressed by epithelial tissues. IFN-ω with antiviral, antitumor activity and regulating the innate immune response. Able to activate P13K/Akt signaling pathway via binding its receptor IFNAR in cells.
Gene ID 3467
Alternative interferon omega, IFN alpha II-1, IFNW1
Accession P05000

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