| Product name | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Mammals, Bacteria |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:3000-1:10000). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Conjugate | HRP |
| Alternative | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4 and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
| Alternative | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
Fig. Western blot analysis of 2ug His fusion protein with HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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