| Product name | Fluo-4 Calcium Assay Kit |
| Applications notes | Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration. |
| Kit components | •Fluo-4 AM (500×) •Staining Enhancer (500×) •Assay Buffer |
| Usage notes | •If a medium containing serum is used, the esterase in the serum will hydrolyze Fluo-4 AM, thus reducing the effectiveness of Fluo-4 AM in entering cells. In addition, the medium containing phenol red will make the background value slightly higher, and the residual medium should be removed as far as possible before adding the Stain Solution. • Ionomycin is an ionic vector with affinity for calcium. The increase in green fluorescence intensity can be observed within a few seconds after Ionomycin treatment. It is recommended to use laser confocal microscope to continuously take photos to detect the change of intracellular calcium concentration. |
| Storage instructions | Stored at -20℃ for 12 months, protected from light |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Fig.Fluo-4 Calcium Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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