| Product name | EliKine™ Mouse GLP-1 Competitive ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | EliKine™ Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1(GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value. |
| Detection method | Colorimetric |
| SampleType | Serum, Plasma, Cell culture supernatants, Cell lysates, and cell culture supernatant |
| Assay type | Competitive |
| Assay duration | It depends on the experience of the operation person. |
| Alternative | Glucagon Like Peptide 1;GLP-1;GLP1 |
| Kit components | •Mouse GLP-1 Microplate •Mouse GLP-1 Standard (lyophilized) •Sample Diluent (5×) •Assay Buffer (5×) •Biotin Conjugated Mouse GLP-1 Detect Antibody (100×) •Streptavidin-HRP (100×) •HRP Substrate（TMB） •Stop Solution •Wash Buffer (20×) •Plate Covers |
| Features & Benefits | EliKine™ Mouse GLP-1 Competitive ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GLP-1. |
| Calibration range | 24.69 pg/mL-2,000 pg/mL |
| Limit of detection | 8.23 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GLP-1 is a peptide hormone released from intestinal L-cells upon nutrient consumption. It bindsthe GLP-1 receptorin the pancreas and displays various antidiabetic effects by potentiating glucose-induced secretion of insulin from pancreatic β-cells, increasing insulin expression. EliKine™ Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1(GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value. |
| Alternative | Glucagon Like Peptide 1;GLP-1;GLP1 |
Fig.1. Human Trypsinogen activation peptide (TAP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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