| Product name | EliKine™ Human FSH ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for FSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person. |
| Alternative | Follicle stimulating hormone alpha chain; Follicle Stimulating Hormone Beta; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin alpha chain; Follitropin beta chain; Follitropin subunit beta; FSH A; FSH alpha; FSH B; FSH beta; FSHA; FSHB |
| Kit components | • Human FSH microplate • Human FSH standard • Human FSH detect antibody • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Human FSH ELISA Kit has high sensitivity and excellent specificity for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed. |
| Calibration range | 2 IU/L-70 IU/L |
| Limit of detection | 1.0 IU/L |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for |
| Storage instructions | The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis. |
| Gene ID | 2488 |
| Alternative | Follicle stimulating hormone alpha chain; Follicle Stimulating Hormone Beta; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin alpha chain; Follitropin beta chain; Follitropin subunit beta; FSH A; FSH alpha; FSH B; FSH beta; FSHA; FSHB |
| Accession | P01225 |
Fig.1. Human FSH Standard Curve.
Fig.2. EliKine™ Human FSH ELISA Kit is a sandwich ELISA to quantitate FSH in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.