| Product name | EliKine™ Human CCL3 ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human CCL3 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL3 in samples. An antibody specific for CCL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL3 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3 |
| Kit components | • Human CCL3 microplate • Human CCL3 standard • Human CCL3 detect antibody • Avidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Human CCL3 ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL3. No significant cross-reactivity or interference between Human CCL3 and analogues was observed. |
| Calibration range | 31.25 pg/mL- 2000 pg/mL |
| Limit of detection | 16 pg/Ml |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has proinflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease |
| Gene ID | 6348 |
| Alternative | G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3 |
| Accession | P10147 |
Fig.1. Human CCL3 Standard Curve.
Fig.2. EliKine™ Human CCL3 ELISA Kit is a sandwich ELISA to quantitate CCL3 in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.