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CheKine™ Mirco Triose-Phosphate Isomerase (TPI) Activity Assay Kit

CheKine™ Mirco Triose-Phosphate Isomerase (TPI) Activity Assay Kit

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Specification

Product name CheKine™ Mirco Triose-Phosphate Isomerase (TPI) Activity Assay Kit
SampleType Plant tissues
Alternative TPI

Product Properties

Kit components •Extraction BufferI
•Extraction Buffer Ⅱ
•Reagent I
•Reagent Ⅱ
•Reagent Ⅲ
•Reagent Ⅳ
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Triose-phosphateisomerase (TPI) in plant chloroplasts is an important enzyme involved in calvin cycle in photosynthesis. Acting on the transformation between glyceraldehyde phosphate and dihydroxyacetone phosphate, dihydroxyacetone phosphate can quickly penetrate the chloroplast envelope and enter the cytoplasm, where it is gradually transformed into sucrose. CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit can detect plant tissues In this kit, TPI converts dihydroxyacetone phosphate into glyceraldehyde-3-phosphate, and glyceraldehyde-3-phosphate and NAD react with glyceraldehyde-3-phosphate dehydrogenase to generate glyceric acid-3-phosphate and NADH. The absorbance change at 340 nm reflects the activity of TPI.
Alternative TPI

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