| Product name | CheKine™ Mirco Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase (PFP) Activity Assay Kit |
| SampleType | Animal and Plant tissues, Cells, Plasma, Serum or other Liquid samples |
| Alternative | PFP Transferase (PFP) |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent V •Reagent VI |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Phosphofructokinase (PFP, EC 2.7.1.90) is a cytoplasmic enzyme that is widely present in plant tissues. It catalyzes the reversible conversion between fructose-6-phosphate and fructose-1,6-bisphosphate and plays a crucial role in carbon metabolism during photosynthesis. CheKine™ Mirco Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase (PFP) Activity Assay Kit provides a simple, convenient, and rapid method for measuring PFP activity in animal and plant tissues, cells, serum (plasma), or other liquid samples. The principle of the assay is based on PFP catalyzing the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, which is then converted to glyceraldehyde-3-phosphate by aldolase and triose phosphate isomerase. Subsequently, glyceraldehyde-3-phosphate dehydrogenase and NADH catalyze the formation of 3-phosphoglycerate, NAD, and phosphate. The change in absorbance at 340 nm reflects the activity of PFP. |
| Alternative | PFP Transferase (PFP) |
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.