| Product name | CheKine™ Mirco Ploygalacturonase (PG) Activity Assay Kit |
| SampleType | Plant Tissue, Bacteria, Fungus, Liquid samples |
| Alternative | PG |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Ploygalacturonase (PG) (EC 3.2.1.15) is a kind of pectinase, which is widely found in plants, bacteria and fungi. It catalyses the decomposition of polygalacturonic acid, which plays an important role in fruit softening, pollen pollination, seed maturation and organ shedding. In addition, when pathogenic bacteria infect host plants, they can secrete polygalacturonase to degrade host cell wall, thus leading to the development of disease. CheKine™ Micro Ploygalacturonase (PG) Activity Assay Kit can be used to detect biological samples such as plant tissue, bacteria,fungus, liquid samples. In the kit, PG hydrolyzes polygalacturonic acid to produce galaturonate, which has a reducing aldehyde group, reacts with DNS reagent to produce reddish-brown substance, and has a characteristic absorption peak at 540 nm. The activity of polygalacturonase can be calculated by measuring the change of absorption value at 540 nm. |
| Alternative | PG |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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