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CheKine™ Mirco Pectin Lyase (PL) Activity Assay Kit

CheKine™ Mirco Pectin Lyase (PL) Activity Assay Kit

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Specification

Product name CheKine™ Mirco Pectin Lyase (PL) Activity Assay Kit
SampleType Plant Tissues, Bacteria, Fungi and Culture media
Alternative PL

Product Properties

Kit components •Reagent I
•Reagent Ⅱ
•Reagent Ⅲ
•Standard
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pectin lyase (EC4.2.2.10) is an important component of pectinases, which is a depolymerase that can degrade plant cell walls,leading to the softening or even death of plant tissues. It has a wide range of sources, mainly from microorganisms. It can be used for clarification of fruit juice and wine, improving the yield of fruit juice, purification of plant viruses, bleaching of pulp, and biorefining of textiles. It has potential application value in reducing environmental pollution and lowering energy consumption. The CheKine™ Mirco Pectin Lyase Activity Assay Kit offers a simple, convenient, and rapid method for detecting PL activity, suitable for samples such as plant tissues, bacteria, fungi, and culture media. Its principle involves pectin lyase acting on the α-1,4-glycosidic bonds in pectin to produce unsaturated oligogalacturonic acids with an unsaturated bond between C4 and C5 at the reducing end, which have a characteristic absorption peak at 235 nm.
Alternative PL

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