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CheKine™ Mirco Carotenoid Content Assay Kit

CheKine™ Mirco Carotenoid Content Assay Kit

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Specification

Product name CheKine™ Mirco Carotenoid Content Assay Kit
SampleType Plant tissues
Alternative Carotenoid

Product Properties

Kit components •Extraction Buffer
•Reagent I
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Carotenoids are lipid-soluble compounds with nutritional properties that provide natural pigments to plants and animals. They are important antioxidants and have the ability to convert into essential vitamins. Carotenoids can prevent damage to cells, tissues, and genes, enhance the body's immune system, resist infections, reduce the risk of cancer, and protect the heart. CheKine™ Mirco Carotenoid Content Assay Kit provides a simple, convenient, and rapid method for measuring carotenoid content, suitable for plant tissue samples. The principle involves carotenoids in plants are found in various yellow plastids or chromoplasts, such as in yellow leaves, yellow flowers, yellow and red fruits, and yellow roots. Samples are extracted using solvents to separate and extract carotenoids. Carotenoids have a specific absorption peak at 440±10 nm. In higher plants and algal microorganisms, chloroplasts also contain carotenoids. Carotenoids primarily absorb blue and violet light, while chlorophyll a and b absorb both red and blue-violet light. Therefore, for tissues containing chloroplasts, to exclude the interference from chlorophyll a and b on carotenoids, the content of chlorophyll a and b is first calculated using empirical formulas. Then, the carotenoid content is determined. For tissues without chlorophyll, the carotenoid content can be directly calculated using the empirical extinction coefficient of carotenoids.
Alternative Carotenoid

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