| Product name | CheKine™ Mirco Calcium Content Assay Kit |
| SampleType | Animal and Plant tissues, Cells, Plasma, Serum or other Liquid samples |
| Alternative | Calcium |
| Kit components | • Reagent I •Reagent Ⅱ •Reagent Ⅲ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Calcium (calcium) is typically the most abundant mineral in the bodies of mammals and is involved in regulating numerous cellular life processes. It is also one of the most important intracellular regulatory factors. Calcium can exist in two forms: as free ions or as complexes with bound calcium ions, such as calcium phosphate and calcium carbonate, which are components of bone tissue. A wide range of physiological processes, including muscle contraction, cell adhesion, hormone/neurotransmitter release, glycogen metabolism, cell proliferation/differentiation, blood coagulation, neural or synaptic transmission, and bone structure support, are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems can lead to the development of various diseases. Although 99% of the calcium in the human body is found in bones and teeth, clinical attention is often focuse on blood calcium levels, known as serum calcium. Blood calcium levels are primarily regulated by parathyroid hormone, which controls the storage or release of calcium from bones to prevent hypercalcemia or hypocalcemia. Calcium in serum exists primarily in three forms: approximately 50% as free calcium ions, about 45% as protein-bound calcium, and about 5% as calcium complexes (mainly calcium citrate). Calcium levels are typically inversely related to phosphorus levels. CheKine™ Mirco Calcium Content Assay Kit provides a simple, convenient, and rapid method for detecting calcium ion content, suitable for animal and plant tissues, cells, serum (plasma) or other Liquid samples. The principle is based on the reaction of calcium ions with o-cresolphthalein complexone under alkaline conditions to form a purple complex. The calcium ion content can be quantitatively determined by measuring the absorbance at 575 nm. |
| Alternative | Calcium |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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