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CheKine™ Mirco Acid Phosphatase (ACP) Activity Assay Kit

CheKine™ Mirco Acid Phosphatase (ACP) Activity Assay Kit

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Specification

Product name CheKine™ Mirco Acid Phosphatase (ACP) Activity Assay Kit
SampleType Animal and Plant Tissues, Serum (Plasma)
Alternative ACP

Product Properties

Kit components •Extraction Buffer
•Reagent I
•Reagent Ⅱ
•Reagent Ⅲ
•Standard
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ACP (Acid Phosphatase) catalyzes the hydrolysis of phosphomonoesters into inorganic phosphate under acidic conditions, commonly found within lysosomes of macrophages. ACP is frequently employed as an auxiliary diagnostic tool for prostate cancer. CheKine™ Micro Acid Phosphatase (ACP) Activity Assay Kit provides a simple, convenient, and rapid method for determining ACP activity, applicable to a variety of samples including plant and animal tissues, serum, and plasma. Its principle relies on the ACP-catalyzed hydrolysis of sodium phenyl phosphate to produce phenol in an acidic milieu; subsequently, phenol reacts with 4-aminoantipyrene and potassium ferricyanide to form a red quinone derivative which exhibits characteristic light absorption at 510 nm. By measuring the rate of increase in absorbance at 510 nm, the ACP activity can be calculated.
Alternative ACP

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