Product name | CheKine™ Micro Sucrose Synthetase (SS) Activity Assay Kit |
SampleType | Plant tissue |
Alternative | Sucrose Synthetase (SS) |
Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent Ⅴ |
Features & Benefits | The verified samples are complete in type and easy to operate. |
Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Sucrose is the primary form by which photosynthetic products from sources such as leaves are transported to “sink” organs. Sucrose synthase (EC 2.4.1.13) is a bidirectional enzyme that catalyzes both sucrose synthesis and its breakdown, making it one of the key enzymes in sucrose metabolism. The study of the synthetic direction activity of SS-Ⅱis of significant importance for understanding plant sucrose synthesis. CheKine™ Micro Sucrose Synthetase (SS) Activity Assay Kit enables the detection of sucrose synthase activity in plant tissues. The assay principle relies on SS-II catalyzing a reaction between free fructose and the glucose donor UDPG to form sucrose. Subsequently, the formed sucrose reacts with resorcinol, causing a color change. This color change exhibits a characteristic absorption peak at 480 nm. The intensity of the color is directly proportional to the enzyme activity, allowing for the quantification of sucrose synthase activity within the sample. |
Alternative | Sucrose Synthetase (SS) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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