| Product name | CheKine™ Micro Nitrate Reductase (NR) Activity Assay Kit |
| SampleType | Plant tissues, bacteria, or cells |
| Alternative | Nitrate Reductase (NR) |
| Kit components | •Inducer Stock Solution •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent Ⅴ |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Nitrate reductase (NR, EC1.7.1.3) exists widely in plants. It is not only the key enzyme for the conversion of nitrate nitrogen to ammonia nitrogen, but also an inducing enzyme, which has an effect on the yield and quality of crops. CheKine™ Micro Nitrate Reductase (NR) Activity Assay Kit allows for the detection of nitrate reductase activity in plant tissues, bacterial, or cellular samples. The principle behind this assay involves NR catalyzing the reduction of nitrate to nitrite, following the reaction:NO3ˉ+NADH+H＋ →NO2ˉ+NAD＋ +H2O. The resulting nitrite, under acidic conditions, then reacts quantitatively with p-amino benzenesulfonic acid and α-naphthylamine to produce a red azo compound. This red azo compound exhibits a maximum absorption peak at 540 nm, which can be measured by spectrophotometry, thereby enabling the quantification of nitrate reductase activity in the sample. |
| Alternative | Nitrate Reductase (NR) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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