| Product name | CheKine™ Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample. |
| Kit components | • Ferrous Salt • H2O2 •Salicylic Acid • Free-HSA Negative Control |
| Features & Benefits | • simple, sensitive, rapid detection method. • compatible to various biological samples such as plasma, serum,bacteria, animal /plant tissues and cells and other biological fluids. • Provides detailed sample preparation , results calculation methods and comprehensive results display. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. • If the OD value exceeds 1.3, the sample needs to be diluted. • The sample is hemolyzed or has a dark color and needs to be compared with itself. The specific method is to add 200 µL of the diluted sample and 30 µL of Extraction Buffer (1x) to UV microplate, incubate at 37°C for 5 min, record the absorbance at 340 nm, and subtract the OD value of the self-control from the measurement well during calculation. • Serum, plasma and tissue samples are recommended to be diluted 5 times with Extraction Buffer (1x) for testing. • The Reagent I and Reagent II are stored in sealed condition. |
| Storage instructions | Storage at 4°C. Kit has a storage time of 12 months from receipt. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Abbkine CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample. |
Fig.CheKine™ Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method).
Author：Liu M, Wu H, Li Q Publication name：Journal of Colloid and Interface Science IF：9.90
Author：Liu M, Wu H, Li Q, et al Publication name：Journal of Colloid and Interface Science IF：9.9
Author： Lin S, Zhang Q, Li S Publication name：CELL PROLIFERATION IF：8.755
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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