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CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit

CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit

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Specification

Product name CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit
SampleType Animal and plant tissues, bacteria and cells, serum or plasma
Alternative Asparagine Synthetase (AS)

Product Properties

Kit components •Reagent I
•Reagent Ⅱ
•Reagent Ⅲ
•Reagent Ⅳ
•Reagent Ⅴ
•Reagent VI
•Standard
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Asparagine synthetase (AS) is a widely distributed enzyme in living organisms belonging to the class of amino transferases. It catalyzes the transfer of an amine group from glutamine to aspartic acid. When a plant is subjected to ammonia toxicity, the formation of asparagine serves as a detoxification mechanism. CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit is designed to quantify asparagine synthetase activity in animal and plant tissues, bacterial and cellular samples, as well as in serum (plasma). The assay principle relies on the AS enzyme's ability to catalyze the hydrolysis of L-asparagine into L-aspartic acid and ammonia. By employing Nessler's reagent to detect the rate of ammonia accumulation, the enzymatic activity of AS can be determined.
Alternative Asparagine Synthetase (AS)

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