| Product name | CheKine™ Micro α-glucosidase(α-GC) Activity Assay Kit |
| Applications notes | The CheKine™ α-Glucosidase Activity Assay Kit (micro assay) provides a simple method for the detection of glycogen synthase activity in biological samples such as plant and animal tissues, cells and serum (plasma). In the assay, p-nitrophenol, which is present in the sample, has a maximum absorption peak at 400 nm, and α-GC activity is calculated by determining the rate of increase in absorbance value. |
| Kit components | •Extraction Buffer •Reagent I •Reagent II •Reagent III •Standard |
| Features & Benefits | •Determines alpha-glucosidase activity in animal and plant tissues, cells, and serum (plasma). •Detailed methods for sample preparation and calculation of results are provided. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | α-Glucosidase (3.2.1.20, EC) widely exists in animals, plants, microorganisms and cultured cells. It catalyzes the hydrolysis of α-glucosidic bonds between aryl or hydrocarbyl groups and glycosyl groups to produce glucose, which is not only related to the relaxation or reinforcement of cell walls, but also closely related to cell recognition and the production of some signal molecules. α-GC decomposes p-nitrobenzene-α-D-glucopyranoside to generate p-nitrophenol, which has the maximum absorption peak at 400 nm. α-GC activity is calculated by measuring the rising rate of absorbance value. |
Fig. CheKine™ Micro α-glucosidase(α-GC) Activity Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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