| Product name | CheKine™ Micro Glycogen synthase(GCS) Activity Assay Kit |
| Applications notes | The CheKine™ Glycogen Synthase Activity Assay Kit (micro assay) provides a simple method for the detection of glycogen synthase activity in biological samples such as animal tissues, cells and bacteria. In the assay, the GCS present in the sample catalyzes the formation of glycogen and UDP from UDPG and glucose residues, and pyruvate kinase and lactate dehydrogenase further catalyze the formation of NAD+ from NADH sequentially, and the rate of decrease of NADH is measured at 340 nm, which is indicative of GCS activity. |
| Kit components | • Extraction Buffer • Reagent I •Reagent II •Reagent III •Reagent IV •Reagent V |
| Features & Benefits | •Determines glycogen synthase activity in animal tissues, cells, and bacteria. • Detailed methods for sample preparation and calculation of results are provided. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Glycogen synthase (GCS) adds the glycosyl of UDPG to the original glycogen or the non-reducing end of glycogen protein, and connects them with α-1,4 glycosidic bonds. GCS is the rate-limiting enzyme in the process of glycogen synthesis in animals, and it is also the main target enzyme of insulin, which plays an important role in the process of glucose metabolism and maintaining the relative stability of blood sugar. GCS catalyzes UDPG and glucose residues to generate glycogen and UDP, and pyruvate kinase and lactate dehydrogenase further catalyze NADH to generate NAD+ in turn. The activity of GCS can be reflected by measuring the decline rate of NADH at 340 nm. |
Fig. CheKine™ Micro Glycogen synthase(GCS) Activity Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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