| Product name | Bcl-2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Bcl-2 around the non-phosphorylation site of T69 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | BCL2; Apoptosis regulator Bcl-2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | BCL2 encodes an integral outer mitochondrial membrane protein (BCL2, apoptosis regulator) that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
| Gene ID | 596 |
| Alternative | BCL2; Apoptosis regulator Bcl-2 |
| Others | Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein. |
| Accession | P10415 |
Fig.1. Western Blot analysis of various cells using Bcl-2 Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of 3T3 cells using Bcl-2 Polyclonal Antibody diluted at 1:1000.
Author：Kim, Myunghee, et al. Publication name：Nutrients IF：5.9
Author： Publication name：Evidence -Based Complementary and Alternative Medicine IF：2.65
Author：E Jin, Y Pei, T Liu, et al Publication name：Food and Agricultural Immunology IF：2.07
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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