Flow products are coming , millions of sample packs wait for your trail use!
Have you ever encountered or are facing the following puzzles in the research of proteomics: The ECL color reagent is not sensitive enough, and the target protein with low expression cannot detect the signal? The color rendering effect is not good, and it disappears in a moment? The fresh reagents bought are inactivated after being stored for four months for a few months? Is the antibody easy to inactivate after dilution? Can't be recycled? Commercially available ordinary antibody diluents can only dilute the primary antibody but not the secondary antibody? The diluted antibody has a high background for protein detection, which is difficult to eliminate? For many friends with selection difficulties, which index should be the most appropriate internal reference…
Abbkine Holiday Arrangement of 2021 Spring Festival
Blood calcium and ionized calcium, how to check how to measure, one box in one step
You may sometimes experience tetany and trembling In life, and you may also experience muscle weakness. All of this makes us think, why? Welcome to today's forum, the hot cause of blood calcium! So what is blood calcium? What is ionized calcium? What is the connection with our body, do we need to understand? The answer is yes. Almost all blood calcium exists in plasma, and blood calcium mainly refers to plasma calcium. Plasma calcium has ionized calcium and bound calcium. Only ionized calcium directly plays a physiological role. It is in dynamic equilibrium with bound calcium and is affected by blood pH. Blood calcium level is related to many important physiological functions, too high or low will affect…
Common problems of Abbkine recombinant protein dissolution
The correct way to dissolve freeze-dried powder: Step 1: Centrifuge before opening the cover. During transportation, the freeze-dried powder will adhere to the tube wall or the tube cover due to external forces. Before opening the cover, the freeze-dried powder will be collected at the bottom of the tube by centrifugal operation to avoid loss and waste. Centrifuge at 10000-12000rpm, 30s or 3000-3500rpm, 5min. Step 2: After centrifugation, add the provided buffer to the freeze-dried powder, mix gently with a pipette tip, and resuspend to a concentration of not less than 100 ug/ml. (For example, the recombinant protein with the specification of 100 ug should be dissolved by adding 1 ml of buffer). A: Use the provided buffer to resuspend…
Abbkine Price Adjustment Announcement
Abbkine Product Package Upgrade Announcement
How to quickly, accurately and stably label the subcellular structure of living cells and fixed cells
TraKine™ Pro is a series of long-term super-resolution cell staining imaging combination, used to mark the subcellular structure of living cells and fixed cells, through the design sequence to achieve specific binding with the target Marker protein gene sequence, and with high brightness and The resolution of the fluorescence is directly coupled. The unique fluorescent dyes of the TraKine™ Pro series cover the spectrum from ultraviolet to near-infrared. This time I emphatically recommend the TraKine™ Pro series of selected popular research targets, which occupy a place in the scientific research field, including: KTC4100-1 …
Cytokines, what you need and what we have
Cytokines are small proteins (~5–20 kDa) that are crucial in controlling the growth and activity of other immune system cells and blood cells. Cytokines have been shown to be involved in autocrine, paracrine and endocrine signaling as immunomodulating agents. When released, they signal the immune system to do its job. (Image source: Google) Cytokine is a general name; other names include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced…
How to draw ELISA standard curve with CurveExpert 1.4 Software
The standard curve of ELISA generally uses special curve fitting tools, such as: CurveExert 1.4. CurveExpert 1.4 is a comprehensive curve fitting system for Windows. XY data can be modelled using a toolbox of linear regression models, nonlinear regression models, interpolation, or splines. Next, we will take Curve Exert1.4 as an example to briefly introduce how to make the standard curve of ELISA: Start "Curve Expert 1.3" software. Enter the OD value of the standard on the X axis and the corresponding concentration value on the Y axis, as shown below: Click the [Run] button (red circle above), and the following dialog box appears: 4. Click the [0K] button, the following two dialog boxes appear, and close the…
CurveExert 1.4 software
Click on the link below to download the CurveExert 1.4 software CurveExpert-Plot drawing
