Product name | Transferrin Polyclonal Antibody |
Immunogen | Synthesized peptide derived from the C-terminal region of human Transferrin |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | ELISA, IHC-P, WB |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications. |
Clonality | Polyclonal |
Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Alternative | TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin |
Formulation | Liquid solution |
Concentration | 1 mg/ml |
Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | TF encodes a glycoprotein with an approximate molecular weight of 76. kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. Serotransferrin may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. |
Gene ID | 7018 |
Alternative | TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin |
Others | Transferrin Polyclonal Antibody detects endogenous levels of Transferrin protein. |
Accession | P02787 |
Observed Band(KD) | 77 |
Fig.1. Western Blot analysis of K562 cells using Transferrin Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of hela using TF Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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